Structural analyses of PCNA from the fungal pathogen Candida albicans identify three regions with species‐specific conformations

An assembly of multiprotein complexes achieves chromosomal DNA replication at the fork. In eukaryotes, proliferating cell nuclear antigen (PCNA) plays a vital role in fork and is essential for viability. PCNA from several organisms, including Saccharomyces cerevisiae , has been structurally characterised. However, structural analyses fungal pathogens are limited. Recently, we have reported that opportunistic pathogen Candida albicans complements functions ScPCNA S. . Still, it only partially rescues loss when yeast cells under genotoxic stress. To understand this further, herein, determined crystal structure CaPCNA compared with existing structures other human PCNA. Our comparative in‐solution small‐angle X‐ray scattering (SAXS) reveal forms stable homotrimer, both solution. It displays noticeable alterations oligomerisation interface, P‐loop hydrophobic pocket regions, suggesting its differential function heterologous system avenues developing specific therapeutics. Databases The PDB SASBDB accession codes 7BUP SASDHQ9, respectively.